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Thermo Fisher
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Jackson Immuno
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Image Search Results
Journal: Bioengineering & Translational Medicine
Article Title: Hyperelastic, shape‐memorable, and ultra‐cell‐adhesive degradable polycaprolactone‐polyurethane copolymer for tissue regeneration
doi: 10.1002/btm2.10332
Figure Lengend Snippet: Hspa1a mediated integrin α5 and αν expression as a PCL‐PU (ISB‐P) cell‐adhesive mechanism with human mesenchymal stem cells. (a) Cell adhesion number, area, and representative images stained with DAPI (blue) and phalloidin (green) for the nuclei and F‐Actin of hMSCs on PCL and ISB‐P at 1 h after seeding ( n = 8). (b) hMSCs treated with si‐Hapa1a for Hspa1a gene knockdown showed lower number and area of adherent cells than the si‐Ctrl group ( n = 8). (c)–(d) Integrin functional blocking assays involving cell adhesion on PCL‐PU (ISB‐P). Each integrin subunit was blocked through preincubation with integrin blocking antibody for 15 min at 37°C. Preincubated cells were seeded and cultured onto ISB‐P for 1 h at 37°C. Adhered cells were stained with phalloidin (green) and DAPI (blue) and quantified by ImageJ ( n = 20). The results indicated that integrin α5 and αν are involved in the adhesion of hMSCs to ISB‐P. *** p < 0.0001, n = 8 or 20 by t test or one‐way ANOVA with Dunnett's test.
Article Snippet: ISB‐P films were punched with a 6 mm diameter and sterilized by EO gas. hMSCs (2 × 10 4 cells/100 μl) were preincubated with 10 μg/ml of
Techniques: Expressing, Adhesive, Staining, Knockdown, Functional Assay, Blocking Assay, Cell Culture
Journal: Bioengineering & Translational Medicine
Article Title: Hyperelastic, shape‐memorable, and ultra‐cell‐adhesive degradable polycaprolactone‐polyurethane copolymer for tissue regeneration
doi: 10.1002/btm2.10332
Figure Lengend Snippet: Summary of optimal PCL‐PU (ISB‐P) characteristics for tissue regeneration. Biodegradable PCL‐PUs were newly synthesized with PCL, HDI and isosorbide or its derivatives. From four candidates, ISB‐P was selected as optimal polymer in tissue regeneration or engineering. Currently optimized PCL‐PU (ISB‐P) is hyperelastic (~1100% strain × ~50 MPa), shape‐memorable (room temperature (RT) ↔ ~37°C), ultra‐cell‐adhesive (better than bare PCL) via HSP mediated integrin activation, and tissue regenerative with great biocompatibility against tissue and major organs. HDI, 1,6‐hexamethylene diisocyanate; HSP, heat shock protein; PCL, polycaprolactone; RT, room temperature; U, urethane group.
Article Snippet: ISB‐P films were punched with a 6 mm diameter and sterilized by EO gas. hMSCs (2 × 10 4 cells/100 μl) were preincubated with 10 μg/ml of
Techniques: Synthesized, Polymer, Adhesive, Activation Assay