function-blocking antibodies against integrin subunits β3 (25e11) Search Results


90
Millipore mab1957z (β3; clone 25e11)
Mab1957z (β3; Clone 25e11), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mab1957z (β3; clone 25e11)/product/Millipore
Average 90 stars, based on 1 article reviews
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90
Millipore mab1976z (αvβ3; clone lm609)
Mab1976z (αvβ3; Clone Lm609), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mab1976z (αvβ3; clone lm609)/product/Millipore
Average 90 stars, based on 1 article reviews
mab1976z (αvβ3; clone lm609) - by Bioz Stars, 2026-03
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90
Millipore mab2021z (αv; clone av1)
Mab2021z (αv; Clone Av1), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mab2021z (αv; clone av1)/product/Millipore
Average 90 stars, based on 1 article reviews
mab2021z (αv; clone av1) - by Bioz Stars, 2026-03
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90
Millipore function-blocking antibodies against integrin subunits β3 (25e11)
Hspa1a mediated <t>integrin</t> α5 and αν expression as a PCL‐PU (ISB‐P) cell‐adhesive mechanism with human mesenchymal stem cells. (a) Cell adhesion number, area, and representative images stained with DAPI (blue) and phalloidin (green) for the nuclei and F‐Actin of hMSCs on PCL and ISB‐P at <t>1</t> h after seeding ( n = 8). (b) hMSCs treated with si‐Hapa1a for Hspa1a gene knockdown showed lower number and area of adherent cells than the si‐Ctrl group ( n = 8). (c)–(d) Integrin functional blocking assays involving cell adhesion on PCL‐PU (ISB‐P). Each integrin subunit was blocked through preincubation with integrin blocking antibody for 15 min at 37°C. Preincubated cells were seeded and cultured onto ISB‐P for 1 h at 37°C. Adhered cells were stained with phalloidin (green) and DAPI (blue) and quantified by ImageJ ( n = 20). The results indicated that integrin α5 and αν are involved in the adhesion of hMSCs to ISB‐P. *** p < 0.0001, n = 8 or 20 by t test or one‐way ANOVA with Dunnett's test.
Function Blocking Antibodies Against Integrin Subunits β3 (25e11), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/function-blocking antibodies against integrin subunits β3 (25e11)/product/Millipore
Average 90 stars, based on 1 article reviews
function-blocking antibodies against integrin subunits β3 (25e11) - by Bioz Stars, 2026-03
90/100 stars
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86
Thermo Fisher yo yo 1
Hspa1a mediated <t>integrin</t> α5 and αν expression as a PCL‐PU (ISB‐P) cell‐adhesive mechanism with human mesenchymal stem cells. (a) Cell adhesion number, area, and representative images stained with DAPI (blue) and phalloidin (green) for the nuclei and F‐Actin of hMSCs on PCL and ISB‐P at <t>1</t> h after seeding ( n = 8). (b) hMSCs treated with si‐Hapa1a for Hspa1a gene knockdown showed lower number and area of adherent cells than the si‐Ctrl group ( n = 8). (c)–(d) Integrin functional blocking assays involving cell adhesion on PCL‐PU (ISB‐P). Each integrin subunit was blocked through preincubation with integrin blocking antibody for 15 min at 37°C. Preincubated cells were seeded and cultured onto ISB‐P for 1 h at 37°C. Adhered cells were stained with phalloidin (green) and DAPI (blue) and quantified by ImageJ ( n = 20). The results indicated that integrin α5 and αν are involved in the adhesion of hMSCs to ISB‐P. *** p < 0.0001, n = 8 or 20 by t test or one‐way ANOVA with Dunnett's test.
Yo Yo 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/yo yo 1/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
yo yo 1 - by Bioz Stars, 2026-03
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90
Biomeda corporation gel/mount
Hspa1a mediated <t>integrin</t> α5 and αν expression as a PCL‐PU (ISB‐P) cell‐adhesive mechanism with human mesenchymal stem cells. (a) Cell adhesion number, area, and representative images stained with DAPI (blue) and phalloidin (green) for the nuclei and F‐Actin of hMSCs on PCL and ISB‐P at <t>1</t> h after seeding ( n = 8). (b) hMSCs treated with si‐Hapa1a for Hspa1a gene knockdown showed lower number and area of adherent cells than the si‐Ctrl group ( n = 8). (c)–(d) Integrin functional blocking assays involving cell adhesion on PCL‐PU (ISB‐P). Each integrin subunit was blocked through preincubation with integrin blocking antibody for 15 min at 37°C. Preincubated cells were seeded and cultured onto ISB‐P for 1 h at 37°C. Adhered cells were stained with phalloidin (green) and DAPI (blue) and quantified by ImageJ ( n = 20). The results indicated that integrin α5 and αν are involved in the adhesion of hMSCs to ISB‐P. *** p < 0.0001, n = 8 or 20 by t test or one‐way ANOVA with Dunnett's test.
Gel/Mount, supplied by Biomeda corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gel/mount/product/Biomeda corporation
Average 90 stars, based on 1 article reviews
gel/mount - by Bioz Stars, 2026-03
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90
Jackson Immuno cy-5-conjugated goat anti-mouse igg
Hspa1a mediated <t>integrin</t> α5 and αν expression as a PCL‐PU (ISB‐P) cell‐adhesive mechanism with human mesenchymal stem cells. (a) Cell adhesion number, area, and representative images stained with DAPI (blue) and phalloidin (green) for the nuclei and F‐Actin of hMSCs on PCL and ISB‐P at <t>1</t> h after seeding ( n = 8). (b) hMSCs treated with si‐Hapa1a for Hspa1a gene knockdown showed lower number and area of adherent cells than the si‐Ctrl group ( n = 8). (c)–(d) Integrin functional blocking assays involving cell adhesion on PCL‐PU (ISB‐P). Each integrin subunit was blocked through preincubation with integrin blocking antibody for 15 min at 37°C. Preincubated cells were seeded and cultured onto ISB‐P for 1 h at 37°C. Adhered cells were stained with phalloidin (green) and DAPI (blue) and quantified by ImageJ ( n = 20). The results indicated that integrin α5 and αν are involved in the adhesion of hMSCs to ISB‐P. *** p < 0.0001, n = 8 or 20 by t test or one‐way ANOVA with Dunnett's test.
Cy 5 Conjugated Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cy-5-conjugated goat anti-mouse igg/product/Jackson Immuno
Average 90 stars, based on 1 article reviews
cy-5-conjugated goat anti-mouse igg - by Bioz Stars, 2026-03
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96
Jackson Immuno goat anti mouse igg
Hspa1a mediated <t>integrin</t> α5 and αν expression as a PCL‐PU (ISB‐P) cell‐adhesive mechanism with human mesenchymal stem cells. (a) Cell adhesion number, area, and representative images stained with DAPI (blue) and phalloidin (green) for the nuclei and F‐Actin of hMSCs on PCL and ISB‐P at <t>1</t> h after seeding ( n = 8). (b) hMSCs treated with si‐Hapa1a for Hspa1a gene knockdown showed lower number and area of adherent cells than the si‐Ctrl group ( n = 8). (c)–(d) Integrin functional blocking assays involving cell adhesion on PCL‐PU (ISB‐P). Each integrin subunit was blocked through preincubation with integrin blocking antibody for 15 min at 37°C. Preincubated cells were seeded and cultured onto ISB‐P for 1 h at 37°C. Adhered cells were stained with phalloidin (green) and DAPI (blue) and quantified by ImageJ ( n = 20). The results indicated that integrin α5 and αν are involved in the adhesion of hMSCs to ISB‐P. *** p < 0.0001, n = 8 or 20 by t test or one‐way ANOVA with Dunnett's test.
Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti mouse igg/product/Jackson Immuno
Average 96 stars, based on 1 article reviews
goat anti mouse igg - by Bioz Stars, 2026-03
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Image Search Results


Hspa1a mediated integrin α5 and αν expression as a PCL‐PU (ISB‐P) cell‐adhesive mechanism with human mesenchymal stem cells. (a) Cell adhesion number, area, and representative images stained with DAPI (blue) and phalloidin (green) for the nuclei and F‐Actin of hMSCs on PCL and ISB‐P at 1 h after seeding ( n = 8). (b) hMSCs treated with si‐Hapa1a for Hspa1a gene knockdown showed lower number and area of adherent cells than the si‐Ctrl group ( n = 8). (c)–(d) Integrin functional blocking assays involving cell adhesion on PCL‐PU (ISB‐P). Each integrin subunit was blocked through preincubation with integrin blocking antibody for 15 min at 37°C. Preincubated cells were seeded and cultured onto ISB‐P for 1 h at 37°C. Adhered cells were stained with phalloidin (green) and DAPI (blue) and quantified by ImageJ ( n = 20). The results indicated that integrin α5 and αν are involved in the adhesion of hMSCs to ISB‐P. *** p < 0.0001, n = 8 or 20 by t test or one‐way ANOVA with Dunnett's test.

Journal: Bioengineering & Translational Medicine

Article Title: Hyperelastic, shape‐memorable, and ultra‐cell‐adhesive degradable polycaprolactone‐polyurethane copolymer for tissue regeneration

doi: 10.1002/btm2.10332

Figure Lengend Snippet: Hspa1a mediated integrin α5 and αν expression as a PCL‐PU (ISB‐P) cell‐adhesive mechanism with human mesenchymal stem cells. (a) Cell adhesion number, area, and representative images stained with DAPI (blue) and phalloidin (green) for the nuclei and F‐Actin of hMSCs on PCL and ISB‐P at 1 h after seeding ( n = 8). (b) hMSCs treated with si‐Hapa1a for Hspa1a gene knockdown showed lower number and area of adherent cells than the si‐Ctrl group ( n = 8). (c)–(d) Integrin functional blocking assays involving cell adhesion on PCL‐PU (ISB‐P). Each integrin subunit was blocked through preincubation with integrin blocking antibody for 15 min at 37°C. Preincubated cells were seeded and cultured onto ISB‐P for 1 h at 37°C. Adhered cells were stained with phalloidin (green) and DAPI (blue) and quantified by ImageJ ( n = 20). The results indicated that integrin α5 and αν are involved in the adhesion of hMSCs to ISB‐P. *** p < 0.0001, n = 8 or 20 by t test or one‐way ANOVA with Dunnett's test.

Article Snippet: ISB‐P films were punched with a 6 mm diameter and sterilized by EO gas. hMSCs (2 × 10 4 cells/100 μl) were preincubated with 10 μg/ml of function‐blocking antibodies against integrin subunits α1 (FB12), α2 (P1E6), α3 (P4C2), α4 (P4C2), α5 (P1D6), α6 (NKI‐GOH3), β1 (6S6), β2 (CD18), and β3 (25E11; Millipore, Bedford, MA) in alpha MEM separately for 15 min at 37°C in a humidified atmosphere of 5% CO 2 .

Techniques: Expressing, Adhesive, Staining, Knockdown, Functional Assay, Blocking Assay, Cell Culture

Summary of optimal PCL‐PU (ISB‐P) characteristics for tissue regeneration. Biodegradable PCL‐PUs were newly synthesized with PCL, HDI and isosorbide or its derivatives. From four candidates, ISB‐P was selected as optimal polymer in tissue regeneration or engineering. Currently optimized PCL‐PU (ISB‐P) is hyperelastic (~1100% strain × ~50 MPa), shape‐memorable (room temperature (RT) ↔ ~37°C), ultra‐cell‐adhesive (better than bare PCL) via HSP mediated integrin activation, and tissue regenerative with great biocompatibility against tissue and major organs. HDI, 1,6‐hexamethylene diisocyanate; HSP, heat shock protein; PCL, polycaprolactone; RT, room temperature; U, urethane group.

Journal: Bioengineering & Translational Medicine

Article Title: Hyperelastic, shape‐memorable, and ultra‐cell‐adhesive degradable polycaprolactone‐polyurethane copolymer for tissue regeneration

doi: 10.1002/btm2.10332

Figure Lengend Snippet: Summary of optimal PCL‐PU (ISB‐P) characteristics for tissue regeneration. Biodegradable PCL‐PUs were newly synthesized with PCL, HDI and isosorbide or its derivatives. From four candidates, ISB‐P was selected as optimal polymer in tissue regeneration or engineering. Currently optimized PCL‐PU (ISB‐P) is hyperelastic (~1100% strain × ~50 MPa), shape‐memorable (room temperature (RT) ↔ ~37°C), ultra‐cell‐adhesive (better than bare PCL) via HSP mediated integrin activation, and tissue regenerative with great biocompatibility against tissue and major organs. HDI, 1,6‐hexamethylene diisocyanate; HSP, heat shock protein; PCL, polycaprolactone; RT, room temperature; U, urethane group.

Article Snippet: ISB‐P films were punched with a 6 mm diameter and sterilized by EO gas. hMSCs (2 × 10 4 cells/100 μl) were preincubated with 10 μg/ml of function‐blocking antibodies against integrin subunits α1 (FB12), α2 (P1E6), α3 (P4C2), α4 (P4C2), α5 (P1D6), α6 (NKI‐GOH3), β1 (6S6), β2 (CD18), and β3 (25E11; Millipore, Bedford, MA) in alpha MEM separately for 15 min at 37°C in a humidified atmosphere of 5% CO 2 .

Techniques: Synthesized, Polymer, Adhesive, Activation Assay